广西玉米纹枯病菌培养性状及其致病力研究

从广西玉米主产区的8个县市秋玉米的纹枯病病株中分离出8个玉米纹枯病菌株,经生物性状的观察和菌丝融合群测定表明,它们都属于立枯丝核菌AGk-1-IA融合群;腐生定殖能力和致病力测定表明,菌株GX4、GX1的腐生定殖能力和致病力最强。     

禽流感血清抗体与卵黄抗体消长规律比较研究

应用禽流感病毒二价（H5、H9）油乳剂灭活疫苗免疫160日龄非免疫蛋鸡，免疫接种后分别于第0、7、14、21、28、35 d采集相应的鸡蛋和血清，采用血凝抑制(HI)试验监测血清及卵黄中H5、H9抗体的消长规律，结果表明：H5、H9血清抗体于免疫后7 d时开始产生，14 d时到中等水平，21 d时达到最高值，一直维持到35 d之后；而H5、H9卵黄抗体与血清抗体水平相比相对滞后7 d左右；卵黄抗体与血清抗体在28 d时均达到高峰值并一直维持到35 d之后，28 d后血清和卵黄抗体达到相同水平。本研究为临床上以禽流感卵黄抗体检测替代血清抗体检测及以后禽流感高免卵黄抗体的研究提供了依据和数据。     

毛乌素沙地3种豆科植物根际AM 真菌生态分布研究

通过对毛乌素沙地草木樨(Melilotus suaveolens)、胡枝子(Leapedeza bicolor)和沙打旺(Astragalus adsurgens)等3种豆科植物根际土壤样品和根样的采集和分析,研究了3种植物根际AM真菌的群落组成和生态分布.试验结果表明,在已分离出的3属28种AM真菌中,球囊霉属(Glomus)种类占绝对优势;3种豆科植物都有较高的AM真菌定殖率和孢子密度,但不同种之间或同一种植物在不同样地之间的AM真菌种类和分布差异显著.AM真菌泡囊定殖率和总定殖率与土壤速效N有显著负相关,其定殖强度可能主要取决于宿主植物和AM真菌的相互选择性.     

烟草青枯病内生拮抗菌的筛选、鉴定及其防效测定

 植物体内的大量微生物,在进化的过程中,由于长期生活在植物特定部位,因而与植物形成了一种特殊的关系,这些微生物在植物体内大量繁殖和扩散,有望成为生物防治有潜力的微生物。内生细菌是植物体内大量存在的微生物之一,具有在植株体内分布广、定殖能力强、防效好以及增殖和扩散快等优点,因而成为发展前景很好的植物病害生防菌。     

Complementary host–pathogen genetic analyses of the role of fumonisins in the Zea mays–Gibberella moniliformis interaction

Zea mays often is colonized with the fungus Gibberella moniliformis, which produces fumonisin toxins. The role of fumonisins in seedling colonization and blight was studied using complementary genetic analyses of host and pathogen. Only one of two fumonisin B1 (FB1)-insensitive maize backcross lines was more resistant than the FB1-sensitive parent to seedling blight, indicating that the increase in FB1-insensitivity was not associated with an increase in resistance. FB1-producing and nonproducing isogenic fungal strains did not differ in ability to cause seedling blight, but the FB1-producing strain was more effective in systemic colonization of seedlings in reciprocal strain challenge tests. Together, these and previous results indicate that the role of fumonisins depends on complex environmental and genetic contexts in this host–pathogen interaction.     

抗香蕉枯萎病菌拮抗菌的鉴定及其定殖

通过逐步提高药物浓度方法,获得了抗利福平400μg/ml、对香蕉枯萎病菌拮抗活性稳定的T2WF和W10标记菌株。初步确定两菌株为芽孢杆菌属细菌。采用灌根接种法,在香蕉苗根际土和香蕉苗体内定殖结果表明,拮抗菌液接种浓度为5×104cfu/ml,两菌株均可从香蕉根际土和香蕉体内分离到,并在香蕉体内定殖和传导。在香蕉根际土、根部、球茎和假茎中,T2WF标记菌的菌量高峰分别出现在接种后5、5、11和3d,分别为707、437、273、117cfu/mg;而W10标记菌的菌量高峰分别出现在接种后5、3、5和5d,分别为784、260、253、110cfu/mg。两菌株在香蕉根际土和香蕉体内1~15d的消长动态均有一个共同的“由升到降”趋势。从数量和数量变动幅度上看,香蕉根部的菌量明显高于茎部的菌量。     

油菜内生生防菌BY-2在油菜体内的定殖与对油菜菌核病的防治作用

 从油菜植株体内分离出的内生细菌BY-2,经过生物学鉴定为枯草芽孢杆菌(Bacillus subtilis)。用BY-2回接油菜,重新分离到具有BY-2相同形态特征和抑制病原真菌能力的内生菌株。油菜接种后的第10 d,体内的BY-2菌数达(2.24~9.02)×10³ cfu/g鲜植株,25 d仍然保持在(3.13~8.59)×10³ cfu/g鲜植株。BY-2与油菜核盘菌[Sclerotinia sclerotiorum(Lib.) de Bary]对峙培养可以形成直径为3.1 cm的抑菌圈;可使油菜核盘菌菌丝细胞浓缩变短,细胞壁破裂,原生质外溢,从而抑制真菌生长发育;同时还能抑制菌核的萌发,抑制率达60%~70%;在油菜离体叶片试验中,BY-2对菌核病的防治效果达100%。     

Stimulation of the bronchus-associated lymphoid tissue of goats and its effect on in vitro colonization by Pasteurella haemolytica

Twenty goats of about 7 months of age were divided into five groups. The goats in groups 1 and 2 were exposed once, using an intranasal spray to 2 ml of an inoculum containing 10⁶ colony-forming units/ml of living or dead Pasteurella haemolytica A2, respectively. The goats in groups 3 and 4 were similarly exposed twice at a 2-week interval. Group 5 was the untreated control. The number and size of the bronchus-associated lymphoid tissue (BALT) in goats exposed twice to either living or dead organisms were significantly (p>0.05) increased compared with those exposed once and with the unexposed control. In vitro colonization by living P. haemolytica A2 onto the lung tissue in which the BALT had been stimulated by two exposures of either living or dead organisms was significantly (p>0.05) reduced. The study indicates that stimulation of the respiratory mucosal immunity may prevent P. haemolytica A2 infection.     

基于村民委员会候选人产生方式的调查与分析

文章以L村和X村村民委员会换届选举中村委会候选人的产生方式为例,指出在村民委员会候选人确立方式上存在着行政权力与自治权之间的冲突,分析原因并提出解决路径,即在立法方面明确村民自治的内函与地位,行政部门应充分为村民自治创造空间,尝试无候选人选举等。     

罗城县雷暴天气活动规律及其灾害防御

利用广西罗城县1980—2009年的雷暴资料,通过统计分析雷暴活动的状况,年变化趋势,季节、月和日分布特征,找出了雷暴活动的时间和空间上的变化规律,分析了1980—2009年来雷暴发生影响的天气系统,计算出各天气条件下雷暴发生的概率。为今后雷电活动监测和分析提供依据,为防灾减灾工作提供参考,同时也比较具体地提出了灾害的防御措施,从而为加强罗城县雷暴防御提供参考。